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Do you mean to include the preamp in the line-out to line-in loop?
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Yep. Since the preamp is part of the microphones response, then to compensate for its response imperfections, it is a good idea to offset it by including it in the soundcard cals correction file. You have to use the line-in and line-out of the preamp and do a bit of fussing with the levels to get the soundcard cal to setup correctly, but it's worth it if it has a poor upper or lower frequency response. Once you've created the soundcard cal file, you remove it from the loop and use it as normal.
brucek